Laboratory Services

IGH/BCL2 Translocation

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Updated Test Information:

Test Description
IGH/BCL2 Translocation

t(14;18); 14;18 translocation; IGH-BCL2 fusion; IGH-BCL2 translocation; Follicular Lymphoma; Diffuse Large Cell Lymphoma; Lymphoproliferative disorders

General Information

Probes: IGH (14q32.33)/BCL2 (18q21.33)

Disease(s): Follicular Lymphoma; Diffuse Large Cell Lymphoma; Lymphoproliferative disorders

Specimen Requirements

Bone Marrow Aspirate and Peripheral Blood
2-5 mL Sodium Heparin tube (preferred)
EDTA tube (acceptable)

Formalin fixed paraffin embedded (FFPE) tissue blocks or
2 unstained slides (4-5 microns thick sections)
1 H&E slide for pathology review (required)

Fixed-cell pellet from cytogenetic analysis slides with metaphase and/or interphase nuclei. (If sending slides please send at least 2 slides for testing)

Additional Processing Details

Transport to the testing lab at room temp is acceptable. Do not allow sample to overheat or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Required Information

Complete the Incyte Diagnostics hematology requisition with the patient's demographics and relevant clinical history. Label the specimen containers with patient's full name, second unique identifier, and specimen site/source. Please provide a copy of the patient's complete blood count (CBC) report.

Unacceptable Specimen Conditions

Specimen containers without two (2) patient identifiers, specimens submitted in inappropriate collection media, frozen specimens.

Department (code)



Fluorescence in-Situ Hybridization (FISH)

Estimated TAT

Heme samples: 3-5 working days from receipt.

FFPE samples: 4-7 working days from receipt.

Testing Schedule



Heme samples: 7 days after release of final report.

FFPE Samples:
Blocks:10 years
H&E and IHC Slides: 10 years
IF Slides: 30 days

CPT Code(s)

88377x1 manual

Performing Lab

Incyte Diagnostics

Additional Information

Clinical Significance: The IGH/BCL2 translocation is present in approximately 70-95% of cases of follicular lymphoma and 30% of diffuse large B-cell lymphomas. Analysis for t(14;18)(q32;q21) or variant BCL2 translocations is useful in distinguishing these lymphomas from other types of NHL.

If Flow Cytometry studies are also being ordered, it is preferable to submit an EDTA (purple top) tube specifically for Flow Cytometry. If only one tube can be submitted, please submit one sodium heparin (green top) tube for both FISH and Flow Cytometry studies.