Test Directory

HLA-B27, Blood

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Updated Test Information:

Test Description
HLA-B27, Blood

B27; Histocompatibility Antigen; Human Leukocyte Antigen; Leukocyte Antigens; LY27; Spondylitis; W-27 (HLA-B27)

Test ID
General Information

Useful for diagnosis of ankylosing spondylitis, juvenile rheumatoid arthritis, and Reiter syndrome

Specimen Type

Whole Blood

Specimen Requirements

Lavender top (EDTA)

Additional Processing Details

Specimen must arrive within 96 hours of draw


Ambient: 4 days

Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.


  • Orders received for both this test and SSO1 / HLA Class I Molecular Phenotype, Blood or DISI / HLA Class I Molecular Typing Disease Association (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap. This HLA-B27 test is best used alone if a particular disease such as ankylosing spondylitis is under consideration

  • Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing


Flow Cytometry

Estimated TAT

2-6 days

Testing Schedule



7 days

CPT Code(s)


Additional Information
Minimum Sample Volume

1 mL

Reference Range

An interpretive report will be provided

Performing Lab


LOINC Code(s)

26028-1, 69052-9