Laboratory Services

CD4 Count for Immune Monitoring, Blood

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Updated Test Information:

Test Description
CD4 Count for Immune Monitoring, Blood

CD4 Count, CD8 Count, Flow Cytometry, Helper Suppressor Ratio, Immune Competence, Immune Status-Flow Cytometry, Immunodeficiency Panel-Flow Cytometry, Quantitative CD4 and CD8, T Cell, T Cells, T Lymphocyte Surface Markers, T-Cell Surface Markers, T-Cells, T-Helper/T-Suppressor-Flow Cytometry, T4/T8 Helper Suppressor Ratio, AIDS (Acquired, Immune Deficiency Syndrome)

Test ID
General Information

Serial monitoring of CD4 T cell count in HIV-positive patients

Useful for follow-up and diagnostic evaluation of primary cellular immunodeficiencies, including severe combined immunodeficiency

T-cell immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized

Assessment of T-cell immune reconstitution post hematopoietic cell transplantation

Early screening of gross quantitative anomalies in T cells in infection or malignancies

Container Type


Specimen Type

Whole Blood EDTA

Specimen Requirements

3mL Lavender top EDTA. Pink EDTA acceptable.  Collect Monday-Thursday only.  Sample must be received by Mayo Laboratories within 72 hours of collection

Specimen Collection / Processing Instructions

Send specimen in original tube. Do not aliquot.

Minimum Sample Volume



Room Temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Unacceptable Specimen Conditions

Gross hemolysis, gross lipemia


Flow Cytometry, Single Platform (CD3, CD4, CD8, CD45)

Estimated TAT

1-2 days

Testing Schedule

Monday through Sunday

Test Includes

CD45 Total Lymph Count, % CD3 (T Cells), CD3 (T Cells), % CD4 (T Cells), CD4 (T Cells), % CD8 (T Cells), CD8 (T Cells), 4/8 Ratio

CPT Code(s)

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

Reference Range

The appropriate age-related reference values will be provided on the report.

Performing Lab

Mayo Clinic

LOINC Code(s)


Additional Information