Laboratory Services

5-Hydroxyindoleacetic Acid, 24 Hour, Urine

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Updated Test Information:

Test Description
5-Hydroxyindoleacetic Acid, 24 Hour, Urine
Synonym(s)

5-HIAA (5-Hydroxyindolacetic Acid); 5-OH-Indoleacetic Acid; Carcinoid Syndrome; HIAA (Hydroxyindoleacetic Acid); Serotonin Metabolite

Test ID
HIAA
General Information

Useful for biochemical diagnosis and monitoring of intestinal carcinoid syndrome

Specimen Type

24-Hour Urine

Specimen Collection / Processing Instructions

  • Patient Preparation:

  • Some medications could interfere with test results. The ordering provider should decide if any medications should be stopped and when they should be restarted. If clinically feasible, discontinue the following medications at least 48 hours prior to beginning collection until collection is complete:

    • Acetaminophen (Tylenol or generic versions)

    • Aspirin

    • Antihistamines

    • Cough syrups

    • Cold and flu medications



  • For 48 hours prior to beginning collection until collection is complete, the patient should limit intake of the following to one serving per day:

    • Fruits

    • Vegetables

    • Nuts

    • Caffeinated beverages or foods



  • Collection Instructions:

  • Collect a 24-hour urine specimen

  • Add 25 mL of 50% acetic acid as preservative at start of collection

  • Use 15 mL of 50% acetic acid for children <5 years old.

  • The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.

  • See Collection and Transportation for 24-Hour Urine Specimens for complete details.

Required Information

  • 24-hour volume is required

  • Patient's age and gender are required

Stability

  • Refrigerated (preferred): 56 days

  • Frozen: 365 days

Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Limitations

  • Intake of food with a high content of serotonin (avocados, dates, eggplant, all fruit [including bananas, cantaloupe, grapefruit, kiwifruit, melons, pineapple, plantains, plums], all nuts [including hickory nuts, butternuts, pecans, walnuts], and tomatoes and tomato products) within 48 hours of the urine collection could result in falsely elevated 5-hydroxyindoleacetic acid (5-HIAA) excretion

  • Numerous drugs affect the excretion of 5-HIAA by different mechanisms, including increased serotonin synthesis, metabolism, and release and inhibition of uptake. The following medications can interfere with 5-HIAA results

    • Acetaminophen (Tylenol or generic versions)

    • Aspirin

    • Antihistamines -Cough syrups

    • Cold and flu medications

    • Patient should also avoid caffeinated beverages such as tea and coffee or caffeinated foods for 48 hours before and during specimen collection.



Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Estimated TAT

2-4 days

Testing Schedule

Monday-Friday

Retention

7 days

CPT Code(s)

83497

Additional Information
Minimum Sample Volume

1 mL

Reference Range

5HIAA (mg/24Hr)

99th Percentile Cutoff

Age (years) Male Female Age (years) Male Female
< 2 ≤ 2.3 ≤ 2.7 49 ≤ 9.1 ≤ 7.7
2 ≤ 2.6 ≤ 3.0 50 ≤ 9.2 ≤ 7.7
3 ≤2.9 ≤ 3.2 51 ≤ 9.3 ≤ 7.8
4 ≤ 3.2 ≤ 3.4 52 ≤ 9.4 ≤ 7.8
5 ≤ 3.6 ≤ 3.6 53 ≤ 9.5 ≤ 7.9
6 ≤ 3.9 ≤ 3.8 54 ≤ 9.6 ≤ 8.0
7 ≤ 4.2 ≤ 4.0 55 ≤ 9.7 ≤ 8.1
8 ≤ 4.5 ≤ 4.2 56 ≤ 9.7 ≤ 8.1
9 ≤ 4.8 ≤ 4.5 57 ≤ 9.8 ≤ 8.2
10 ≤ 5.1 ≤ 4.7 58 ≤ 9.8 ≤ 8.3
11 ≤ 5.3 ≤ 4.9 59 ≤ 9.8 ≤ 8.3
12 ≤ 5.6 ≤ 5.2 60 ≤ 9.9 ≤ 8.3
13 ≤ 5.8 ≤ 5.4 61 ≤ 9.9 ≤ 8.3
14 ≤ 6.1 ≤ 5.6 62 ≤ 9.9 ≤ 8.4
15 ≤ 6.3 ≤ 5.7 63 ≤ 10.0 ≤ 8.4
16 ≤ 6.4 ≤ 5.9 64 ≤ 10.0 ≤ 8.4
17 ≤ 6.6 ≤ 6.0 65 ≤ 10.0 ≤ 8.4
18 ≤ 6.7 ≤ 6.0 66 ≤ 10.1 ≤ 8.5
19 ≤ 6.8 ≤ 6.1 67 ≤ 10.1 ≤ 8.5
20 ≤ 6.9 ≤ 6.1 68 ≤ 10.1 ≤ 8.5
21 ≤ 6.9 ≤ 6.2 69 ≤ 10.2 ≤ 8.5
22 ≤ 7.0 ≤ 6.2 70 ≤ 10.2 ≤ 8.5
23 ≤ 7.0 ≤ 6.2 71 ≤ 10.2 ≤ 8.6
24 ≤ 7.1 ≤ 6.3 72 ≤ 10.2 ≤ 8.6
25 ≤ 7.2 ≤ 6.3 73 ≤ 10.1 ≤ 8.5
26 ≤ 7.2 ≤ 6.3 74 ≤ 10.1 ≤ 8.5
27 ≤ 7.3 ≤ 6.4 75 ≤ 10.1 ≤ 8.6
28 ≤ 7.4 ≤ 6.4 76 ≤ 10.0 ≤ 8.6
29 ≤ 7.5 ≤ 6.5 77 ≤ 10.0 ≤ 8.6
30 ≤ 7.5 ≤ 6.6 78 ≤ 10.0 ≤ 8.6
31 ≤ 7.6 ≤ 6.6 79 ≤ 10.0 ≤ 8.6
32 ≤ 7.7 ≤ 6.7 80 ≤ 9.9 ≤ 8.7
33 ≤ 7.7 ≤ 6.8 81 ≤ 9.9 ≤ 8.7
34 ≤ 7.8 ≤ 6.8 82 ≤ 9.9 ≤ 8.7
35 ≤ 7.9 ≤ 6.9 83 ≤ 9.9 ≤ 8.7
36 ≤ 7.9 ≤ 6.9 84 ≤ 9.9 ≤ 8.7
37 ≤ 8.0 ≤ 7.0 85 ≤ 9.8 ≤ 8.6
38 ≤ 8.1 ≤ 7.0 86 ≤ 9.8 ≤ 8.5
39 ≤ 8.2 ≤ 7.0 87 ≤ 9.7 ≤ 8.4
40 ≤ 8.2 ≤ 7.1 88 ≤ 9.7 ≤ 8.3
41 ≤ 8.3 ≤ 7.1 89 ≤ 9.5 ≤ 8.1
42 ≤ 8.4 ≤ 7.2 90 ≤ 9.4 ≤ 7.9
43 ≤ 8.5 ≤ 7.3 91 ≤ 9.2 ≤ 7.6
44 ≤ 8.6 ≤ 7.4 92 ≤ 9.0 ≤ 7.4
45 ≤ 8.7 ≤ 7.4 93 ≤ 8.8 ≤ 7.1
46 ≤ 8.8 ≤ 7.5 94 ≤ 8.7 ≤ 7.0
47 ≤ 8.9 ≤ 7.6 ≥ 95 ≤ 8.6 ≤ 6.9
48 ≤ 9.0 ≤ 7.6      

 

Reference Range File View/Download File
Performing Lab

Mayo

LOINC Code(s)

1695-6, 13362-9, 3167-4