Laboratory Services

17-Hydroxypregnenolone, Serum

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Updated Test Information:

Test Description
17-Hydroxypregnenolone, Serum
Test ID
17OHP
General Information

Useful for:



  • Ancillary test for congenital adrenal hyperplasia (CAH), particularly when 21-hydroxylase and 11-hydroxylase deficiency have been ruled out

  • Confirmation of 3-beta-hydroxy dehydrogenase (3-beta-HSD) deficiency

  • Evaluation of females with hirsutism or infertility

Specimen Type

Serum

Specimen Requirements

  • Preferred: Red top tube

  • Acceptable: Serum Gel

Stability

  • Frozen (preferred):  28 days

  • Refrigerated:  28 days

Limitations

At birth, the hypothalamic-pituitary-adrenal axis and the hypothalamic-pituitary gonadal axis are activated and adrenal and sex steroid levels are high. In preterm infants, the elevations can be even more pronounced due to illness and stress. As a result, preterm infants may occasionally have 17-hydroxypregnenolone levels of up to 9,799 ng/dL. Term infants (0-28 days) will have levels <3,104 ng/dL. These then fall over the following 2 years to prepubertal levels of <277 ng/dL.

Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Estimated TAT

2-6 days

Testing Schedule

Monday, Tuesday, Thursday

Retention

14 days

CPT Code(s)

84143

Additional Information
Minimum Sample Volume

0.5 mL

Reference Range

 

Age Males: Range (ng/dL) Females:Range (ng/dL)
Premature (26-28 weeks) 1219 - 9799 1219 - 9799
Premature (29-36 weeks) 346 - 8911 346 - 8911
Full term (1-5 months) 229 - 3104 229 - 3104
6 months-364 days 221 - 1981 221 - 1981
1-2 years 35 - 712 35 - 712
3-6 years 0 - 277 0 - 277
7-9 years 0 - 188 0 - 213
10-12 years 0 - 393 0 - 399
13-15 years 35 - 465 0 - 408
16-17 years 32 - 478 0 - 424
≥ 18 years 55 - 455 31 - 455
TANNER STAGES Males: Range (ng/dL) Females:Range (ng/dL)
Stage I 0 - 209 0 - 236
Stage II 0 - 356 0 - 368
Stage III 0 - 451 0 - 431
Stage IV - V 35 - 478 0 - 413

 

 

 

Performing Lab

Mayo

LOINC Code(s)

6765-2