Laboratory Services

Testosterone, Total by Mass Spectrometry, Serum

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Updated Test Information:

Test Description
Testosterone, Total by Mass Spectrometry, Serum
Synonym(s)

TESTG, UOW4068

Test ID
TESTG
General Information

This method uses liquid-liquid extraction and liquid chromatography-tandem mass spectrometry to quantify the concentration of testosterone in serum or plasma. The analytical measurement range is 10-1000 ng/dL and the imprecision is 2-4 %CV.

Specimen Type

Serum

Alternate Specimen Type

4mL of blood in a LAVENDER TOP

Pediatric: LAVENDER TOP MICROTAINER

Specimen Requirements

4mL of blood in a RED TOP

Specimen Collection / Processing Instructions

Centrifuge and separate serum within 24 hours.

Minimum Sample Volume

requested: 1.0mL Serum/Plasma
minimum: 0.5mL Serum/Plasma

Stability

Room Temp: 8 hrs
2 - 8°C: 2 weeks


For long term storage, store frozen at -20°C.

Unacceptable Specimen Conditions

GREEN TOP (heparin plasma), GOLD TOP SST, LIME GREEN TOP SST, ORANGE TOP SST, PEARL TOP (and any other tube type that contains a gel)

Department (code)

Chemistry, University (CHU)

Methodology

Liquid Chromatography-Tandem Mass Spectroscopy

Testing Schedule

Three times each week, results expected in 3-4 days.

CPT Code(s)

84403

Performing Lab

UW Laboratory

LOINC Code(s)