Fetomaternal Bleed, Flow Cytometry, Blood

Fetal Hemoglobin; Fetal Maternal Bleed; Fetal Maternal Erythrocyte Distribution, Blood; Fetal Red Cells for Fetal Maternal Erythrocyte Distribution; Fetal-Maternal Erythrocyte Differentiation; Kleihauer Acid Elution (Fetal RBC's); Kleihauer-Betke; Maternal Erythrocyte Diff

Useful for determining the volume of fetal-to-maternal hemorrhage for the purposes of recommending an increased dose of the Rh immune globulin

EDTA Whole Blood

Lavender EDTA

• Do not centrifuge


• Invert several times to mix blood


• Send specimen in original tube: do not aliquot as aliquoting can adversely affect test results.


• Specimen must arrive within 5 days (preferably 24-72 hours) of collection

• Refrigerated (preferred): 5 days


• Ambient: 5 days

• Gross hemolysis


• Gross lipemia


• Gross icterus


• Clotted

• This test is for the detection of fetal bleed, it should not be used to detect the hereditary persistence of fetal hemoglobin (HPFH) or to detect fetal maternal hemorrhage in a mother with hereditary persistence of fetal hemoglobin.


• Clinical conditions exist that may result in an increased level of fetal hemoglobin-containing red blood cells (RBC), including hereditary persistence of fetal hemoglobin and thalassemia. Such RBC (also referred to as F cells) are detected by this assay. Results must be interpreted with caution in these situations


• Due to differential RBC densities, aliquoting into or out of a sample tube can adversely affect the results.

Flow Cytometry

1 day

Monday-Sunday

88184

≤ 1.5 mL of fetal RBCs in normal adults

Mayo

10331-7, 55730-6, 55731-4, 48767-8

Test Definition: FMB